Vol. 6, Issue 1, Part A (2024)

Diabetes mellitus is one of the most common disorders among all ages. There are two types of diabetes: type 1 and type 2. In type 1 diabetes there will be no insulin secretion therefore, the glucose molecules cannot be metabolized into energy and remain in the blood circulation, resulting in elevated blood glucose levels and a diabetic condition. In type 2 diabetes (T2D) the insulin receptors fail to respond to insulin production and metabolizing the intake of glucose molecules, which leads to diabetic conditions. Diabetes affects an extensive range of physiological and metabolic activities. Triglyceride levels in the blood are a significant indication of the occurrence of diabetes mellitus 2. Diabetes-2 is caused by an unhealthy dietary habit in day-to-day life. Drosophila, known as the Cinderella of genetics due to its genetic resemblance to humans, can be utilized as a model organism to construct the T2D prototype by feeding it with a high-sugar diet for a period. Because of its shorter life cycle and genetic similarities with humans, we can identify and test for physiological and biochemical alterations caused by the high sugar diet that is relative to humans. Berberine chloride, a bioactive molecule found in many edible plant materials, could regulate blood glucose levels and normalize physiological and biochemical abnormalities. It can be used as a medication to treat type 2 diabetes. As this molecule is not hazardous to insects, it can be used to treat HSD-induced flies and examine its potentiality for diabetic conditions. In this study, we investigate the anti-diabetic potential of berberine chloride on the HSD-induced Drosophila melanogaster. To check the protein-ligand interaction in the insulin signaling pathway bioinformatic tools (In-silico) were incorporated to predict the drug binding capacity in a specific site of the targeted protein. The significance of all the test data was verified by using statistical tools.